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pegfp-c1-rela (gfp-p65  (Addgene inc)


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    Structured Review

    Addgene inc pegfp-c1-rela (gfp-p65
    Pegfp C1 Rela (Gfp P65, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp-c1-rela+(gfp-p65/gfp+rela/10__1128_slash_jvi__00400___23-454-77-85
    Average 90 stars, based on 1 article reviews
    pegfp-c1-rela (gfp-p65 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: The Cytomegalovirus M35 Protein Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes by Direct Promoter Binding
    Article Snippet: pGL3basic-IFNβ-Luc ( mIfnb1 - FLuc) consists of the 812 bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene ( ).

    Article Title: The Cytomegalovirus M35 Protein Directly Binds to the Interferon-β Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes
    Article Snippet: pGL3basic-IFN-β-Luc ( mIfnb1 -FLuc) consists of the 812-bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene ( ).

    Luciferase:

    Article Title: The Cytomegalovirus M35 Protein Directly Binds to the Interferon-β Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes.
    Article Snippet: Plasmids. pRL-TK expressing Renilla luciferase under the control of the thymidine kinase (TK) promoter is commercially available (catalog no. E2241, Promega, Walldorf, Germany). pGL3basic-IFN-b-Luc (mIfnb1-FLuc) consists of the 812-bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (92). .. The firefly luciferase reporter plasmid p-125 (hIfnb1-FLuc), consisting of the human Ifnb1 promoter region (2125 to 119), was kindly provided by Takashi Fujita (Kyoto University, Japan) (93). pFLAG-CMV-huIPS1 expressing Flag-MAVS was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (94). pCMVBL IRF35D encoding constitutively active human IRF3 by containing five amino acid substitutions (S396D, S398D, S402D, S404D, and S405D) was kindly provided by John Hiscott (Institut Pasteur Cenci Bolognetti Foundation, Rome, Italy). pEGFP-C1-RelA (GFP-p65, catalog number 23255) is available from Addgene (Watertown, MA, USA). pIRES2-GFP (catalog number 6029-1), pQCXIH, and pQCXIP (catalog number 631516) vectors were purchased from Clontech Laboratories (Mountain View, CA, USA). pcDNA3.11 empty vector (EV; catalog number V790-20) and pcDNA3.1 TOPO EV (catalog number K480001) are from Invitrogen (Thermo Fisher Scientific). pEGFP-C1-hIRF3 encoding human IRF3 N-terminally fused with eGFP (eGFP-IRF3) was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (95). ..

    Plasmid Preparation:

    Article Title: The Cytomegalovirus M35 Protein Directly Binds to the Interferon-β Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes.
    Article Snippet: Plasmids. pRL-TK expressing Renilla luciferase under the control of the thymidine kinase (TK) promoter is commercially available (catalog no. E2241, Promega, Walldorf, Germany). pGL3basic-IFN-b-Luc (mIfnb1-FLuc) consists of the 812-bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (92). .. The firefly luciferase reporter plasmid p-125 (hIfnb1-FLuc), consisting of the human Ifnb1 promoter region (2125 to 119), was kindly provided by Takashi Fujita (Kyoto University, Japan) (93). pFLAG-CMV-huIPS1 expressing Flag-MAVS was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (94). pCMVBL IRF35D encoding constitutively active human IRF3 by containing five amino acid substitutions (S396D, S398D, S402D, S404D, and S405D) was kindly provided by John Hiscott (Institut Pasteur Cenci Bolognetti Foundation, Rome, Italy). pEGFP-C1-RelA (GFP-p65, catalog number 23255) is available from Addgene (Watertown, MA, USA). pIRES2-GFP (catalog number 6029-1), pQCXIH, and pQCXIP (catalog number 631516) vectors were purchased from Clontech Laboratories (Mountain View, CA, USA). pcDNA3.11 empty vector (EV; catalog number V790-20) and pcDNA3.1 TOPO EV (catalog number K480001) are from Invitrogen (Thermo Fisher Scientific). pEGFP-C1-hIRF3 encoding human IRF3 N-terminally fused with eGFP (eGFP-IRF3) was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (95). ..

    Expressing:

    Article Title: The Cytomegalovirus M35 Protein Directly Binds to the Interferon-β Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes.
    Article Snippet: Plasmids. pRL-TK expressing Renilla luciferase under the control of the thymidine kinase (TK) promoter is commercially available (catalog no. E2241, Promega, Walldorf, Germany). pGL3basic-IFN-b-Luc (mIfnb1-FLuc) consists of the 812-bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (92). .. The firefly luciferase reporter plasmid p-125 (hIfnb1-FLuc), consisting of the human Ifnb1 promoter region (2125 to 119), was kindly provided by Takashi Fujita (Kyoto University, Japan) (93). pFLAG-CMV-huIPS1 expressing Flag-MAVS was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (94). pCMVBL IRF35D encoding constitutively active human IRF3 by containing five amino acid substitutions (S396D, S398D, S402D, S404D, and S405D) was kindly provided by John Hiscott (Institut Pasteur Cenci Bolognetti Foundation, Rome, Italy). pEGFP-C1-RelA (GFP-p65, catalog number 23255) is available from Addgene (Watertown, MA, USA). pIRES2-GFP (catalog number 6029-1), pQCXIH, and pQCXIP (catalog number 631516) vectors were purchased from Clontech Laboratories (Mountain View, CA, USA). pcDNA3.11 empty vector (EV; catalog number V790-20) and pcDNA3.1 TOPO EV (catalog number K480001) are from Invitrogen (Thermo Fisher Scientific). pEGFP-C1-hIRF3 encoding human IRF3 N-terminally fused with eGFP (eGFP-IRF3) was kindly provided by Friedemann Weber (Institute of Virology, Justus Liebig University Giessen, Germany) (95). ..



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    Addgene inc pegfp-c1-rela (gfp-p65
    Pegfp C1 Rela (Gfp P65, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp-c1-rela+(gfp-p65/gfp+rela/10__1128_slash_jvi__00400___23-454-77-85
    Average 90 stars, based on 1 article reviews
    pegfp-c1-rela (gfp-p65 - by Bioz Stars, 2026-10
    90/100 stars
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